The questions and answers below relate to the Research Techniques Made Simple article “Enzyme Immunoassay (EIA) and Enzyme-Linked Immunosorbent Assay (ELISA),” published online with the September 2013 issue of JID.
Correct answers appear in bold underline.
Multiple Choice Questions & Answers:
1. Which of the following molecule(s) can be detected by ELISA?
d) all of the above
2. What does a weak color signal in competitive ELISA represent?
a) more antigen in the sample
b) less antigen in the sample
c) less antigen retained on the well
d) both a and c
3. Which of the following is immobilized on the microtiter well in sandwich ELISA?
a) detection antibody
c) capture antibody
d) secondary antibody conjugated to an enzyme
4. What is a major advantage of ELISA in comparison to other biological quantification techniques?
a) detection of a molecule at a low concentration
c) low specificity
d) easily available