The questions and answers below relate to the Research Techniques Made Simple article  “Enzyme Immunoassay (EIA) and Enzyme-Linked Immunosorbent Assay (ELISA),” published online with the September 2013 issue of JID.


Correct answers appear in bold underline.


Multiple Choice Questions & Answers:


1.    Which of the following molecule(s) can be detected by ELISA?

a)     proteins

b)     hormones

c)     antibodies

d)     all of the above


2.     What does a weak color signal in competitive ELISA represent?

a)     more antigen in the sample

b)     less antigen in the sample

c)     less antigen retained on the well

d)     both a and c


3.    Which of the following is immobilized on the microtiter well in sandwich ELISA?

a)    detection antibody

b)    sample

c)    capture antibody

d)    secondary antibody conjugated to an enzyme


4.    What is a major advantage of ELISA in comparison to other biological quantification techniques?

a)    detection of a molecule at a low concentration

b)    inexpensive

c)    low specificity

d)    easily available

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