The questions and answers below relate to the Research Techniques Made Simple (RTMS) entitled “ Genome-Wide Epigenetics” in the June 2013 issue of Journal of Investigative Dermatology. To view the article, go to the RTMS web page.
Questions & Answers
1) Heterochromatin refers to:
A. Actively transcribed regions of DNA to which transcription factors actively bind
B. The core structure around which 147 base pairs of DNA bind
C. Constitutively closed and transcriptionally repressed areas of genome organization
D. Areas of the genome marked by the histone modification H3K4me3
The correct answer is C: Constitutively closed and transcriptionally repressed areas of genome organization.
2) The major role of ChIP-seq experiments lies in:
A. The ability to map DNA methylation patterns across the genome
B. The ability to map the bound histone modifications and other proteins bound to chromatin across the genome
C. The ability to analyze gene expression changes with changes in chromatin modifications
D. The ability to associate single-nucleotide polymorphisms with various disease states
The correct answer is B: The ability to map the bound histone modifications and other proteins bound to chromatin across the genome.