RESEARCH TECHNIQUES MADE SIMPLE: FLUORESCENCE IN SITU HYBRIDIZATION (FISH): Q&A

This quiz relates to the Research Techniques Made Simple article “FLUORESCENCE IN SITU HYBRIDIZATION (FISH)” published in the May 2013 issue of the Journal of Investigative Dermatology.

 

 

Questions:

 

 

1. What does FISH detect?

a. Protein structure abnormalities
b. Specific chromosome copy number aberrations
c. Presence of specific antigens
d. Presence of complement

 

 

2. Where does the FISH probe localize to?

a. Golgi apparatus
b. Cytoplasm
c. Cell membrane
d. Nucleus
3. What is the FISH probe composed of?

a. Proteins
b. Lipids
c. Carbohydrates
d. Nucleic acids

 

 

4. What is the maximum number of FISH probes that can be used in a single experiment?

a. Two
b. Three
c. Four
d. Five

 

 

 

Answers:


1.         The correct answer is b:  Specific chromosome copy number aberrations

2.         The correct answer is d:  Nucleus

3.         The correct answer is d:  Nucleic acids

4.         The correct answer is c:  Four

 

One thought on “RESEARCH TECHNIQUES MADE SIMPLE: FLUORESCENCE IN SITU HYBRIDIZATION (FISH): Q&A

  1. FISH is also used in fundamental research to understand gene positioning. In that case, there are no chromosomic aberrations and question makes no sense.Moreover, the number of probes is only limited by the number of fluorochromes. There are more than 4 and question 4 makes non sense. So please, make it simple but not wrong.

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