For details, we refer you to the article “Research Techniques Made Simple: Immunofluorescence Techniques” by Ian D. Odell1, MD, PhD and Deborah Cook2, MD
Departments of Medicine1 and Pathology2 University of Vermont College of Medicine, Burlington, VT
- What is the purpose of Michel’s solution?
- Fix the tissue before detection
- Cross link cellular components to retain the integrity of cellular structures
- Disrupt plasma membranes to give antibodies access to their target antigens
- Precipitate the immune complexes to preserve antigenicity
- How many additional antibodies are required to detect autoimmune complexes?
- Which of the following techniques is more sensitive than immunofluorescence for the diagnosis of some autoimmune bullous diseases?
- Light microscopy
- Western blot