Research Techniques Made Simple: IMMUNOFLUORESCENCE Q&A

For details, we refer you to the article “Research Techniques Made Simple: Immunofluorescence Techniques” by Ian D. Odell1, MD, PhD and Deborah Cook2, MD

Departments of Medicine1 and Pathology2 University of Vermont College of Medicine, Burlington, VT


  1. What is the purpose of Michel’s solution?
    1. Fix the tissue before detection
    2. Cross link cellular components to retain the integrity of cellular structures
    3. Disrupt plasma membranes to give antibodies access to their target antigens
    4. Precipitate the immune complexes to preserve antigenicity


  1. How many additional antibodies are required to detect autoimmune complexes?
    1. 1
    2. 2
    3. 3
    4. 4


  1. Which of the following techniques is more sensitive than immunofluorescence for the diagnosis of some autoimmune bullous diseases?
    1. Light microscopy
    2. ELISA
    3. Dermatoscope
    4. Western blot


1. D

2. A

3. B

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