RESEARCH TECHNIQUES MADE SIMPLE: CONFOCAL MICROSCOPY Q&A

These questions and answers relate the the article “RESEARCH TECHNIQUES MADE SIMPLE:  INTRODUCTION TO CONFOCAL MICROSCOPY” by Nwaneshiudu et al.  For details see the December 2012 issue of JID.

 

 

QUESTIONS

 

1) Features of confocal microscopy include which of the following:

a. Formation of the focal point of the objective lens on a pinhole to decrease “noise”

b. Increase in the optical resolution and contrast of the image

c. Ability to reconstruct a 3-D image of the specimen

d. Ability to collect serial optical sections from thick specimens

e. All of the above

 

 

2) Which microscope uses a series of moving pinholes on a disk?

a. Programmable array microscope

b. Spinning-disk confocal microscope

c. Scanning transmission electron microscope

d. Phase-contrast microscope

 

 

3)  What is the role of a photomultiplier tube?

a. It collects fluorescence at the dichroic mirror
b. It provides the excitation light

c. It scans the emitted light
d. It detects the emitted light

 

4)  What may be the consequence of using two different fluorescent dyes?

a. Photobleaching

b. Phototoxicity

c. Chromatic and spherical aberration
d. Less detectable photons

 

ANSWERS

1) Features of confocal microscopy include which of the following:

e:  All of the above.

 

 

2) Which microscope uses a series of moving pinholes on a disk?

b:  Spinning-disk confocal microscope

 

3)  What is the role of a photomultiplier tube?

d:  It detects the emitted light

 

4)  What may be the consequence of using two different fluorescent dyes?

c:  Chromatic and spherical aberration

One thought on “RESEARCH TECHNIQUES MADE SIMPLE: CONFOCAL MICROSCOPY Q&A

  1. i found it very useful.
    can you help me in selecting confocal microscope. we have reviewed fv1200 olympus and nikon A1+. both seems to be equally good.

    thankful
    rizwana

    Like

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